Gradient elution for micellar electrokinetic capillary chromatography

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00158a003· OSTI ID:7005557

Balchunas, A T; Sepaniak, M J

A method for performing gradient elution in micellar electrokinetic capillary chromatography is developed. The influence of temperature and mobile phase organic solvent concentration on capacity factors is presented and attributed to changes in phase ratio and solute distribution coefficient. Solvent programming is more effective than temperature programming at adjusting retention due to its greater influence on distribution coefficient. As anticipated, phase ratio changes could not be exploited due to concomitant losses in column efficiency. A stepwise solvent gradient involving increasing concentrations of 2-propanol and Triton-X-100 is used for the separation of a test mixture of derivatized amines.

• Research Organization: Univ. of Tennessee, Knoxville (USA)
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 7005557
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:7; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
AMINES
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMICAL REACTION KINETICS
CHROMATOGRAPHY
DATA
DISTRIBUTION FUNCTIONS
EFFICIENCY
ELECTRODYNAMICS
ELUTRIATION
EXPERIMENTAL DATA
FUNCTIONS
INFORMATION
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
MICELLAR SYSTEMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SOLVENTS
ORGANS
PHASE STUDIES
QUANTITY RATIO
REACTION KINETICS
SEPARATION PROCESSES
SOLUTES
SOLVENTS
TEMPERATURE DEPENDENCE