Separations of compounds of biological and environmental interest by micellar electrokinetic capillary chromatography

Conference · Sat Oct 01 04:00:00 EDT 1988 · Sep. Sci. Technol.; (United States)

OSTI ID:5995775

Balchunas, A T; Swaile, D F; Powell, A C; Sepaniak, M J

Important criteria for the effective separation of compounds of biological or environmental interest by micellar electrokinetic capillary chromatography are discussed. Efficiencies of approximately 100,000 plates/meter are achieved in the separations of samples of derivatized amines, aflatoxins, and hydroxy aromatic compounds. Laser fluorometric detection is shown to be capable of detecting subpicogram injected quantities. Organic solvents such as 2-propanol and acetonitrile are added to the aqueous mobile phases normally used to improve the separation of hydrophobic compounds, impart different selectivities, and provide a means for gradient programming. Column diameter is found to influence efficiency, analysis time, and detection.

• Research Organization: Univ. of Tennessee, Knoxville (USA)
• OSTI ID: 5995775
• Report Number(s): CONF-871038-
• Conference Information: Journal Name: Sep. Sci. Technol.; (United States) Journal Volume: 23:12,13
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
400400 -- Electrochemistry
AFLATOXIN
AMINES
ANTIGENS
AROMATICS
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMISTRY
CHROMATOGRAPHY
DATA
EFFICIENCY
ELECTROCHEMISTRY
EXPERIMENTAL DATA
HYDROXY COMPOUNDS
INFORMATION
MATERIALS
MICELLAR SYSTEMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SOLVENTS
ORGANS
SEPARATION PROCESSES
SOLVENTS
TOXIC MATERIALS
TOXINS