Extension of elution range in micellar electrokinetic capillary chromatography

Journal Article · Fri May 15 04:00:00 EDT 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00137a020· OSTI ID:6541870

Balchunas, A T; Sepaniak, M J

The authors have successfully demonstrated one approach to extend elution range in micellar electrokinetic capillary chromatography (MECC). By silanating the surfaces of fused-silica capillaries with trimethylchlorosilane (TMCS) the authors lowered electroosmotic flow velocity and, more importantly, the net flow velocity of the micelle phase, thereby increasing elution range. This is accomplished at the expense of a loss in column efficiency which primarily results from solute-wall interactions. Addition of 2-propanol to the mobile phase serves to minimize this effect, thereby restoring much of the efficiency lost in the process.

• Research Organization: Univ. of Tennessee, Knoxville
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 6541870
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:10; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
AMINES
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
DATA
EFFICIENCY
ELECTROPHORESIS
ELUTRIATION
EXPERIMENTAL DATA
FLOW RATE
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MICELLAR SYSTEMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
SEPARATION PROCESSES
SOLUTES
SURFACTANTS
VELOCITY