Bile salt surfactants in micellar electrokinetic capillary chromatography: Application to hydrophobic molecule separations

Technical Report · Mon Jan 01 04:00:00 EST 1990

DOI:https://doi.org/10.2172/6287336· OSTI ID:6287336

Cole, R O; Sepaniak, M J ^[1]; Hinze, W L ^[2]; Gorse, J; Oldiges, K ^[3]

---

1. Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry
2. Wake Forest Univ., Winston-Salem, NC (USA). Dept. of Chemistry
3. Baldwin-Wallace Coll., Berea, OH (USA). Dept. of Chemistry

Bile Salt surfactants are used in the micellar electrokinetic capillary chromatography (MECC) separation of various hydrophobic compounds. The use of methanol in the mobile phase allows the separation of previously intractable compounds including polyaromatic hydrocarbons. The effects of methanol on critical micelle concentration is investigated for sodium dodecyl sulfate (SDS) and the bile salt sodium cholate. It is determined that the unique structure of the bile salt micelle is much more tolerant to the addition of organic solvents than SDS, thereby increasing the scope of applications of MECC to include hydrophobic compounds. 30 refs., 9 figs.

• Research Organization: Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 6287336
• Report Number(s): DOE/ER/13613-30; ON: DE91004876
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ALCOHOLS
AROMATICS
BILE ACIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DATA
ELECTROPHORESIS
EXPERIMENTAL DATA
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
METHANOL
MICELLAR SYSTEMS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
SALTS
SEPARATION PROCESSES
STEROIDS
STEROLS
SURFACTANTS