Column efficiency in micellar electrokinetic capillary chromatography

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00130a020· OSTI ID:6814143

Sepaniak, M J; Cole, R O

Experimental factors that influence column efficiency in micellar electrokinetic capillary chromatography were studied. Parameters, such as applied voltage, column dimensions, and concentrations of buffer and surfactant in the mobile phase, are found to influence efficiency. Van Deemter-like plots of plate vs. applied voltage are used to demonstrate the significance of factors which typically cause band dispersion in chromatography and electrophoresis. Dispersion due to resistance to mass transfer in the mobile phase and temperature gradients within the column are shown to be most significant. Plate heights of less than 10..mu..m are possible when experimental parameters are optimized.

• Research Organization: Univ. of Tennessee, Knoxville
• OSTI ID: 6814143
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:3; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
CHROMATOGRAPHY
DATA
DIMENSIONS
EFFICIENCY
ELECTRIC POTENTIAL
ELECTROPHORESIS
EXPERIMENTAL DATA
INFORMATION
MASS TRANSFER
NUMERICAL DATA
OPTIMIZATION
PARAMETRIC ANALYSIS
QUANTITY RATIO
SEPARATION PROCESSES
SURFACTANTS
TEMPERATURE GRADIENTS