/sup 23/Na nuclear magnetic resonance studies of ion transport in the rabbit proximal tubule
Thesis/Dissertation
·
OSTI ID:7002116
/sup 23/Na nuclear magnetic resonance (n.m.r.) spectroscopy was used to measure intracellular sodium levels in suspensions of rabbit proximal tubules. The intracellular sodium signal was resolved by incorporating the paramagnetic shift reagent dysprosium tripolyphosphate into the suspension buffers. This shift reagent was membrane impermeant, and induced a large upfield shift of the n.m.r. signal from the extracellular sodium pool, while leaving the intracellular pool unshifted. Tubule viability and transport characteristics were unimpaired in the presence of the shift reagent provided there was no excess tripolyphosphate, and extra calcium was added to the buffer.
- Research Organization:
- Yale Univ., New Haven, CT (USA)
- OSTI ID:
- 7002116
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BODY
BUFFERS
CHEMICAL SHIFT
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MEMBRANE TRANSPORT
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RABBITS
REAGENTS
RESONANCE
SODIUM 23
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
TRACER TECHNIQUES
TUBULES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BODY
BUFFERS
CHEMICAL SHIFT
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MEMBRANE TRANSPORT
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RABBITS
REAGENTS
RESONANCE
SODIUM 23
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
TRACER TECHNIQUES
TUBULES
VERTEBRATES