Nuclear magnetic resonance studies of intracellular ions in perfused from heart

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Am. J. Physiol.: Heart Circ. Physiol.; (United States)

OSTI ID:6148125

Burnstein, D; Fossel, E T

Intracellular sodium, potassium, and lithium were observed in a perfused frog heart by nuclear magnetic resonance (NMR) spectroscopy. A perfusate buffer containing the shift reagent, dysprosium tripolyphosphate, was used in combination with mathematical filtering or presaturation of the extracellular resonance to separate the intra- and extracellular sodium NMR signals. Addition of 10 ..mu..M ouabain to the perfusate, perfusion with a zero potassium, low-calcium buffer, and replacement of 66% of the perfusate sodium with lithium resulted in changes in the intracellular sodium levels. An increase of 45% in the intracellular sodium was observed when changing the pacing rate from 0 to 60 beats/min (with proportional changes for intermediate pacing rates). The ratio of intracellular potassium to sodium concentration was determined to be 2.3 by NMR, indicating that a substantial amount of the intracellular potassium is undetectable with these NMR method. In addition, intracellular lithium was observed during perfusion with a lithium-containing perfusate.

• Research Organization: Harvard Univ., Cambridge, MA (USA)
• OSTI ID: 6148125
• Journal Information: Am. J. Physiol.: Heart Circ. Physiol.; (United States), Journal Name: Am. J. Physiol.: Heart Circ. Physiol.; (United States) Vol. 21:6; ISSN AJPPD
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
AMPHIBIANS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHARGED PARTICLES
DRUGS
DYSPROSIUM COMPOUNDS
FOURIER TRANSFORMATION
FROGS
GLYCOSIDES
HEART
INTEGRAL TRANSFORMATIONS
IONS
ISOTOPES
LIGHT NUCLEI
LITHIUM 7
LITHIUM COMPOUNDS
LITHIUM ISOTOPES
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PERFUSED TISSUES
PHYSIOLOGY
POTASSIUM 39
POTASSIUM COMPOUNDS
POTASSIUM ISOTOPES
RARE EARTH COMPOUNDS
RESONANCE
SODIUM 23
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
STROPHANTHINS
TISSUES
TRANSFORMATIONS
VERTEBRATES