/sup 35/Cl/sup -/ and /sup 2/H NMR measurement of intracellular water volume in renal proximal tubules

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5020327

Hoffman, D; Kumar, A M; Spitzer, A; Gupta, R K

Knowledge of cell water volume is essential for the calculation of concentrations of intracellular ions and metabolites in kidney tubules. The authors have developed a method which utilizes /sup 35/Cl/sup -/ NMR as a measure of extracellular volume and /sup 2/H NMR in combination with a membrane-impermeant shift reagent Dy(DTPA) as a measure of the ratio of intra- and extracellular water volumes. Measurement of extracellular volume by /sup 35/Cl/sup -/ NMR is possible since intracellular /sup 35/Cl/sup -/ resonance is broadened beyond detection in most cells. The /sup 2/H NMR measurements exploit the fact that only extracellular water is in direct contact with Dy(DTPA). However, rapid exchange of water across the cell membrane results in only a single /sup 2/H/sub 2/O resonance at a chemical shift which is a weighted average of the shifted extra- and unshifted intracellular water resonances. Denoting the extracellular volume as a fraction of the total volume by S/sub o/, and as a fraction of the total water volume by H/sub o/, the fractional cell water content, W = ((1/H/sub o/) -1)/((1/S/sub o/)-1), can be calculated. Rat proximal tubule suspensions were prepared by the method developed in their laboratory. The water content was found to be 76.9 +/- 5% (anti +/- x SD, n = 4) at room temperature and 78.6% at 37/sup 0/C. Increasing extracellular osmolality from 295 to 330 mosm/kg, by addition of mannitol, decreased the water content to 70.8%. The authors results are in good agreement with the previously reported values of 69-82%, obtained by other methods. Thus, NMR is an accurate, noninvasive method for measuring the water content of renal cells.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY
• OSTI ID: 5020327
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHLORINE 35
CHLORINE ISOTOPES
DEUTERIUM
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MEMBRANES
METABOLITES
NMR SPECTRA
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANS
OXYGEN COMPOUNDS
SPECTRA
STABLE ISOTOPES
TUBULES
VOLUME
WATER