Dynamic NMR measurement of volume regulatory changes in Amphiuma RBC Na sup + content

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6947477

Anderson, S E; Adorante, J S; Cala, P M ^[1]

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1. Univ. of California, Davis (USA)

{sup 23}Na nuclear magnetic resonance (NMR) and conventional chemical methods were employed to measure Na{sup +} fluxes in Amphiuma red blood cells (RBC) during volume regulation. Paramagnetic shift reagent (dysprosium triethylenetetraminehexaacetic acid (DyTTHA) and dysprosium tripolyphosphate (Dy(TPP){sub 2})) were used to alter extracellular Na{sup +} magnetic resonance. Data are presented describing {sup 23}Na resonance dependence on shift reagent, sodium and calcium concentration. The authors confirmed that the shift reagents neither enter the cells nor alter intracellular Na{sup +}, K{sup +}, and Cl{sup {minus}} concentrations under control conditions when extracellular calcium was maintained >0.5 mM. They also confirmed that the shift reagent complexes chelate calcium (Dy(TPP){sub 2} much more so than DyTTHA) and that their toxic effects could be alleviated by adjusting calcium in the cell's suspension medium to control levels. In parallel experiments, where volume-activated Na{sup +} fluxes range from 0.3 to 3 mmol Na{sup +}/kg dry cell solid (DCS) {times} minute in cells containing from 30 to 150 mmol Na{sup +}/kg DCS, changes in intracellular sodium measured by {sup 32}Na NMR were within 4% of those measured by conventional destructive methods. Finally, they present data that are consistent with the interpretation that 6 mmol Na{sup +}/kg DCS plus 16% of intracellular Na{sup +} is NMR invisible.

• OSTI ID: 6947477
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
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PROTONS
SODIUM 23
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NUCLEI
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