Dynamic NMR measurement of volume regulatory changes in Amphiuma RBC Na sup + content

Journal Article · Tue Mar 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6947477

Anderson, S E; Adorante, J S; Cala, P M ^[1]

---

1. Univ. of California, Davis (USA)

{sup 23}Na nuclear magnetic resonance (NMR) and conventional chemical methods were employed to measure Na{sup +} fluxes in Amphiuma red blood cells (RBC) during volume regulation. Paramagnetic shift reagent (dysprosium triethylenetetraminehexaacetic acid (DyTTHA) and dysprosium tripolyphosphate (Dy(TPP){sub 2})) were used to alter extracellular Na{sup +} magnetic resonance. Data are presented describing {sup 23}Na resonance dependence on shift reagent, sodium and calcium concentration. The authors confirmed that the shift reagents neither enter the cells nor alter intracellular Na{sup +}, K{sup +}, and Cl{sup {minus}} concentrations under control conditions when extracellular calcium was maintained >0.5 mM. They also confirmed that the shift reagent complexes chelate calcium (Dy(TPP){sub 2} much more so than DyTTHA) and that their toxic effects could be alleviated by adjusting calcium in the cell's suspension medium to control levels. In parallel experiments, where volume-activated Na{sup +} fluxes range from 0.3 to 3 mmol Na{sup +}/kg dry cell solid (DCS) {times} minute in cells containing from 30 to 150 mmol Na{sup +}/kg DCS, changes in intracellular sodium measured by {sup 32}Na NMR were within 4% of those measured by conventional destructive methods. Finally, they present data that are consistent with the interpretation that 6 mmol Na{sup +}/kg DCS plus 16% of intracellular Na{sup +} is NMR invisible.

• OSTI ID: 6947477
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:3; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL SHIFT
ELEMENTARY PARTICLES
ERYTHROCYTES
FERMIONS
FLAME PHOTOMETRY
HADRONS
IONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
MEMBRANE TRANSPORT
MEMBRANES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
PHOTOMETRY
PROTONS
RESONANCE
SODIUM 23
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
VOLUME