NMR monitoring of intracellular sodium in dog and rabbit kidney tubules

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6976160

Boulanger, Y; Vinay, P; Boulanger, M ^[1]

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1. Universite de Montreal, Quebec (Canada)

{sup 23}Na-nuclear magnetic resonance (NMR) was used to monitor intra- and extracellular sodium in suspensions of dog cortical tubules, rabbit cortical tubules, and dog thick ascending limbs. The NMR visibility of the intracellular sodium was determined by comparing the NMR and flame photometry results and by redistributing the sodium ions between the intra- and extracellular compartments using the ionophore nystatin (influx) or sodium substitution for choline in the extracellular fluid (efflux). The intracellular sodium visibility was {approximately}30% for the total sodium and 58% for the transportable sodium. Addition of sodium to sodium-depleted homogenates of dog renal cortex also showed a loss of visibility. The values of the relaxation times T{sub 1} and T{sub 2} were determined but could not be correlated with the visibility measurements. The intracellular sodium concentration in dog cortical tubules incubated in optimal biochemical conditions was estimated at 51 mM was dependent on the extracellular sodium concentration.

• OSTI ID: 6976160
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CHLORIDES
CHLORINE COMPOUNDS
DOGS
FLAME PHOTOMETRY
HALIDES
HALOGEN COMPOUNDS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MEMBRANE TRANSPORT
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANS
PHOTOMETRY
PHYSIOLOGY
RABBITS
RADIOISOTOPES
RELAXATION TIME
RESONANCE
SODIUM 22
SODIUM CHLORIDES
SODIUM COMPOUNDS
SODIUM ISOTOPES
TUBULES
VERTEBRATES
YEARS LIVING RADIOISOTOPES