Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Journal of Bacteriology; (USA)

OSTI ID:6995295

Shiratori, Toshikazu; Inoue, Chihiro; Sugawara, Kazuyuki; Kusano, Tomonobu; Kitagawa, Yoshichika ^[1]

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1. Akita Prefectural College of Agriculture, Ohgata (Japan)

A search of various domestic isolates of Thiobacillus ferrooxidans revealed that some were fairly resistant to mercury ion. A proportion of mercury-resistant clones were able to volatilize mercury, and their corresponding gene was localized not in the plasmid DNA but in chromosomal DNA. This mercury ion resistance gene was cloned in Escherichia coli. E. coli carrying the recombinant plasmid was able to grow in the presence of more than 40 {mu}g of HgCl{sub 2} per ml. Deletion analysis of the recombinant plasmid showed that the entire coding sequence of the mercury ion resistance gene was located within a 2.3-kilobase fragment of the chromosomal DNA from strain E-15. At least two polypeptides (molecular mass, 56 and 16 kDa, respectively) were coded by this fragment.

• OSTI ID: 6995295
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:6; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACTERIA
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
ESCHERICHIA COLI
HYBRIDIZATION
MERCURY COMPOUNDS
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RECOMBINANT DNA
SULFUR-OXIDIZING BACTERIA
THIOBACILLUS FERROXIDANS
TOLERANCE