Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Expression of Thiobacillus ferrooxidans origin of replication in Escherichia coli

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:6007594

A cryptic plasmid from an autotrophically grown arsenic-resistant strain of Thiobacillus ferrooxidans was isolated and cloned into pBR325. The origin of replication of pBR325 was deleted, and the recombinant plasmid was shown to replicate in Escherichia coli, using an origin of replication located on the Thiobacillus plasmid.

Research Organization:
Univ. of Cape Town, Rondebosch, South Africa
OSTI ID:
6007594
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 158:2; ISSN JOBAA
Country of Publication:
United States
Language:
English

Similar Records

Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli
Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Journal of Bacteriology; (USA) · OSTI ID:6995295

Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli
Journal Article · Sat Nov 30 23:00:00 EST 1985 · J. Bacteriol.; (United States) · OSTI ID:5392833

Cloning of a Thiobacillus ferrooxidans plasmid in Escherichia coli
Journal Article · Sat Dec 31 23:00:00 EST 1983 · J. Bacteriol.; (United States) · OSTI ID:6167386