Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli
The glutamine synthetase (GS) gene glnA of Thiobacillus ferrooxidans was cloned on recombinant plasmid pMEB100 which enabled Escherichia coli glnA deletion mutants to utilize (NH/sub 4/)/sub 2/SO/sub 4/ as the sole source of nitrogen. High levels of GS-specific activity were obtained in the E. coli glnA deletion mutants containing the T. ferrooxidans GS gene. The cloned T. ferrooxidans DNA fragment containing the glnA gene activated histidase activity in an E. coli glnA glnL glnG deletion mutant containing the Klebsiella aerogenes hut operon. Plasmid pMEB100 also enabled the E. coli glnA glnL glnG deletion mutant to utilize arginine or low levels of glutamine as the sole source of nitrogen. There was no detectable DNA homology between the T. ferrooxidans glnA gene and the E. coli glnA gene.
- Research Organization:
- Univ. of Cape Town, Rondebosch, South Africa
- OSTI ID:
- 5392833
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 164:3; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
AMMONIUM COMPOUNDS
AMMONIUM SULFATES
ATP
BACILLUS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA-CLONING
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
GLUTAMINE
ISOTOPES
LIGASES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOISOTOPES
SULFATES
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA
THIOBACILLUS FERROXIDANS