Cloning of a Thiobacillus ferrooxidans plasmid in Escherichia coli

Journal Article · Sat Dec 31 23:00:00 EST 1983 · J. Bacteriol.; (United States)

OSTI ID:6167386

Holmes, D S; Lobos, J H; Bopp, L H; Welch, G C

Three separate plasmids of 6, 7, 16, and >23 kilobases were purified from a single clone of Thiobacillus ferrooxidans ATCC 33020 grown in the presence of uranium. The 6.7-kilobase plasmid (pTfl) was cloned separately into the HindIII or BamHI site of Escherichia coli plasmid pBR322. Restriction maps of the recombinant plasmids, termed pTf100 and pTf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between E. coli and T. ferrooxidans. Evidence from restriction enzyme analysis and Southern blot DNA-DNA hybridization indicates that the three native plasmids share little sequence homology.

• Research Organization: General Electric Corp. Research and Development Center, Schenectady, NY
• OSTI ID: 6167386
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 157:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACTINIDES
BACILLUS
BACTERIA
CELL CONSTITUENTS
CLONING
DNA
ELEMENTS
ESCHERICHIA COLI
GENETIC ENGINEERING
METALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
RECOMBINANT DNA
SULFUR-OXIDIZING BACTERIA
THIOBACILLUS FERROXIDANS
TOLERANCE
URANIUM