Effects of chemical and physical carcinogens on viral DNA's: in vivo replication and localization of DNA hot spots

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6994520

Schweikart, K M

Host cell reactivation (HCR) of ultraviolet (UV) and methylmethane sulfonate (MMS)-treated Bacillus subtilis phages 029, SPO2c12, and SPP1 and their DNA's was investigated. The extent of repair among the UV-irradiated phages was compared using quantum yields rather than inactivation constants. The data for these phages assayed in B. subtilis YB864 (uvr/sup -/) indicate that SPO2c12 and SPP1 phages very likely code for an enzyme that aids host cell repair, whereas 029 does not. Repair of transfecting DNA was usually less efficient than repair of DNA form the irradiated intact phage.

• Research Organization: Clemson Univ., SC (USA)
• OSTI ID: 6994520
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
DNA REPLICATION
GENETIC RADIATION EFFECTS
METHYL METHANESULFONATE
GENETIC EFFECTS
BACILLUS SUBTILIS
CARCINOGENS
IN VIVO
ULTRAVIOLET RADIATION
BACILLUS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
ESTERS
MICROORGANISMS
MUTAGENS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
SULFONIC ACID ESTERS
VIRUSES
560130* - Radiation Effects on Microorganisms
560300 - Chemicals Metabolism & Toxicology