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Effects of chemical and physical carcinogens on viral DNA's: in vivo replication and localization of DNA hot spots

Thesis/Dissertation ·
OSTI ID:6994520
Host cell reactivation (HCR) of ultraviolet (UV) and methylmethane sulfonate (MMS)-treated Bacillus subtilis phages 029, SPO2c12, and SPP1 and their DNA's was investigated. The extent of repair among the UV-irradiated phages was compared using quantum yields rather than inactivation constants. The data for these phages assayed in B. subtilis YB864 (uvr/sup -/) indicate that SPO2c12 and SPP1 phages very likely code for an enzyme that aids host cell repair, whereas 029 does not. Repair of transfecting DNA was usually less efficient than repair of DNA form the irradiated intact phage.
Research Organization:
Clemson Univ., SC (USA)
OSTI ID:
6994520
Country of Publication:
United States
Language:
English