Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]

Journal Article · Thu Apr 01 00:00:00 EST 1976 · J. Bacteriol.; (United States)

OSTI ID:7193031

Canosi, U; Siccardi, A G; Falaschi, A; Mazza, G

Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation process of B. subtilis. The results reported in this work demonstrated that the drug was also without effect on the transfection SPP1 or SPO-1 phage DNA (a process that requires a recombination event). The drug was also ineffective on the host cell reactivation of ultraviolet-irradiated SPP1 phage, as well as on transfection with ultraviolet-irradiated DNA of the same phage. HPUra instead markedly reduced the transformation process, as well as transfection, by SPO-1 DNA, but it did not affect the host cell reactivation of SPO-1 phage. In conclusion, whereas the NAL target seems to be specific for replicative DNA synthesis, the HPUra target (i.e., the DNA polymerase III of B. subtilis) seems to be involved also in recombination, but not in the excision repair process. The mutations conferring NAL and HPUra resistance used in this work were mapped by PBS-1 transduction.

• Research Organization: Univ., Pavia
• OSTI ID: 7193031
• Journal Information: J. Bacteriol.; (United States), Vol. 126:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS SUBTILIS
BIOLOGICAL RADIATION EFFECTS
GENE RECOMBINATION
BACTERIOPHAGES
DNA
INHIBITION
ULTRAVIOLET RADIATION
URACILS
AZINES
BACILLUS
BACTERIA
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)