Ability of Bacillus subtilis protoplasts to repair irradiated bacteriophage deoxyribonucleic acid via acquired and natural enzymatic systems

Journal Article · Tue Sep 01 00:00:00 EDT 1981 · J. Bacteriol.; (United States)

OSTI ID:6061238

Yasbin, R E ^[1]; Andersen, B J; Sutherland, B M

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1. Pennsylvania State Univ., Univ. Park

A novel form of enzyme therapy was achieved by utilizing protoplasts of Bacillus subtilis. Photoreactivating enzyme of Escherichia coli was successfully inserted into the protoplasts of B. subtilis treated with polyethylene glycol. This enzyme was used to photoreactivate ultraviolet-damaged bacteriophage deoxyribonucleic acid (DNA). Furthermore, in polyethylene glycol-treated protoplasts, ultraviolet-irradiated transfecting bacteriophage DNA was shown to be a functional substrate for the host DNA excision repair system. Previous results (R.E. Yasbin, J.D. Fernwalt, and P.I. Fields, J. Bacteriol.; 137: 391-396) showed that ultraviolet-irradiated bacteriophage DNA could not be repaired via the excision repair system of competent cells. Therefore, the processing of bacteriophage DNA by protoplasts and by competent cells must be different. This sensitive protoplast assay can be used to identify and to isolate various types of DNA repair enzymes.

• OSTI ID: 6061238
• Journal Information: J. Bacteriol.; (United States), Vol. 147:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACILLUS SUBTILIS
HOST-CELL REACTIVATION
DNA
BIOLOGICAL REPAIR
BACTERIOPHAGES
BIOLOGICAL EFFECTS
ENZYMES
ESCHERICHIA COLI
GLYCOLS
PLANT CELLS
POLYETHYLENE GLYCOLS
THERAPY
ULTRAVIOLET RADIATION
ALCOHOLS
BACILLUS
BACTERIA
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PARASITES
POLYMERS
RADIATIONS
RECOVERY
REPAIR
VIRUSES
550701* - Microbiology- Tracer Techniques