DNA repair in Bacillus subtilis: excision repair capacity of competent cells. [uv radiation]

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Bacteriol.; (United States)

OSTI ID:6509721

Yasbin, R E; Fernwalt, J D; Fields, P I

Competent Bacillus subtilis were investigated for their ability to support the repair of uv-irradiated bacteriophage and bacteriophage DNA. uv-irradiated bacteriophage DNA cannot be repaired to the same level as uv-irradiated bacteriophage, suggesting a deficiency in the ability of competent cells to repair uv damage. However, competent cells were as repair proficient as noncompetent cells in their ability to repair irradiated bacteriophage in marker rescue experiments. The increased sensitivity of irradiated DNA is shown to be due to the inability of excision repair to function on transfecting DNA in competent bacteria. Furthermore, competent cells show no evidence of possessing an inducible BsuR restriction system to complement their inducible BsuR modification enzyme.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 6509721
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 137:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES