Inducible error-prone repair in B. subtilis. Final report, September 1, 1979-June 30, 1981

Technical Report · Mon Jun 01 04:00:00 EDT 1981

DOI:https://doi.org/10.2172/6453839· OSTI ID:6453839

Yasbin, R. E.

The research performed under this contract has been concentrated on the relationship between inducible DNA repair systems, mutagenesis and the competent state in the gram positive bacterium Bacillus subtilis. The following results have been obtained from this research: (1) competent Bacillus subtilis cells have been developed into a sensitive tester system for carcinogens; (2) competent B. subtilis cells have an efficient excision-repair system, however, this system will not function on bacteriophage DNA taken into the cell via the process of transfection; (3) DNA polymerase III is essential in the mechanism of the process of W-reactivation; (4) B. subtilis strains cured of their defective prophages have been isolated and are now being developed for gene cloning systems; (5) protoplasts of B. subtilis have been shown capable of acquiring DNA repair enzymes (i.e., enzyme therapy); and (6) a plasmid was characterized which enhanced inducible error-prone repair in a gram positive organism.

• Research Organization: Pennsylvania State Univ., University Park (USA)
• Sponsoring Organization: USDOE
• DOE Contract Number: AS02-78EV04964
• OSTI ID: 6453839
• Report Number(s): DOE/EV/04964-3; COO-4964-3; ON: DE81027897
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA
ENZYMES
MICROORGANISMS
MUTAGEN SCREENING
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
POLYMERASES
RECOVERY
REPAIR
SCREENING
TRANSFERASES