Inducible error-prone repair in B. subtilis. Final report, September 1, 1979-June 30, 1981
The research performed under this contract has been concentrated on the relationship between inducible DNA repair systems, mutagenesis and the competent state in the gram positive bacterium Bacillus subtilis. The following results have been obtained from this research: (1) competent Bacillus subtilis cells have been developed into a sensitive tester system for carcinogens; (2) competent B. subtilis cells have an efficient excision-repair system, however, this system will not function on bacteriophage DNA taken into the cell via the process of transfection; (3) DNA polymerase III is essential in the mechanism of the process of W-reactivation; (4) B. subtilis strains cured of their defective prophages have been isolated and are now being developed for gene cloning systems; (5) protoplasts of B. subtilis have been shown capable of acquiring DNA repair enzymes (i.e., enzyme therapy); and (6) a plasmid was characterized which enhanced inducible error-prone repair in a gram positive organism.
- Research Organization:
- Pennsylvania State Univ., University Park, PA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AS02-78EV04964
- OSTI ID:
- 6453839
- Report Number(s):
- DOE/EV/04964-3; COO-4964-3; ON: DE81027897
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inducible error-prone repair in B. subtilis. Progress report, September 1, 1981-April 30, 1985
Ability of Bacillus subtilis protoplasts to repair irradiated bacteriophage deoxyribonucleic acid via acquired and natural enzymatic systems
Related Subjects
BACILLUS SUBTILIS
MUTAGEN SCREENING
DNA
BIOLOGICAL REPAIR
PLASMIDS
POLYMERASES
BACILLUS
BACTERIA
BIOLOGICAL RECOVERY
CELL CONSTITUENTS
ENZYMES
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RECOVERY
REPAIR
SCREENING
TRANSFERASES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)