Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Lethal effects of /sup 32/P decay on transfecting activity of Bacillus subtillis phage phie DNA

Journal Article · · Virology; (United States)
Disintegration of /sup 32/P present in the DNA of Bacillus subtilis phage phie (a phage containing double-strand DNA) results in the loss of viability of intact phage as well as transfecting activity of isolated DNA. Only 1/12 of the /sup 32/P disintegrations per phage DNA equivalent inactivities the intact phage while nearly every disintegration inactivates the transfecting DNA. This result provides evidence for a single-strand intermediate in the transfection of B. subtilis by phie DNA.
Research Organization:
Massachusetts Inst. of Tech., Cambridge
OSTI ID:
5297724
Journal Information:
Virology; (United States), Journal Name: Virology; (United States) Vol. 96:1; ISSN VIRLA
Country of Publication:
United States
Language:
English