Repair of nonreplicating UV-irradiated DNA

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6998340

Martin, S J; Hays, J B

Repair of irradiated phage lambda DNA in E. coli has been studied by a repressed-infection system: superinfection of homoimmune lysogenic bacteria; assay for restoration of transcribility to phage-encoded lac genes; extraction of DNA and assay for infectivity in transfection of uvrB/sup -/ recA/sup -/ recB/sup -/ spheroplasts, and for removal of cyclobutane pyrimidine dimers (CBP-dimers) by UV-endonuclease treatment and alkaline sedimentation. In uvr/sup +/ repressed infections with 254-nm irradiated phages (60 J/m/sup 2/) lac transcription was rapidly returned to undamaged levels, concomitant with restoration of infectivity and removal of CBP-dimers. In uvrD/sup -/ cells, the frequency of phage gene inactivation corresponded to the estimated frequency of CBP-dimers per gene. In uvrA/sup -/ bacteria, however, lac expression was only 1/10 to 1/3 of that predicted by the expected frequency of gene inactivation, as if damage elsewhere affected transcription; recovery of infectivity and removal of CBP-dimers was almost completely inhibited. lac/sup +/ and lacUV5 phages, expected to respond oppositely to changes in superhelical density, were constructed as probes for topological changes during DNA repair. The assays for transfection infectivity and CBP-dimer-removal have been extended to studies of repair of UV-irradiated phage DNA injected into oocytes of the frog Xenopus laevis.

• Research Organization: Univ. of Maryland Baltimore County, Catonsville
• OSTI ID: 6998340
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
FROGS
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GERM CELLS
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
OOCYTES
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
TRANSCRIPTION
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES