Effect of diet on triolein absorption in weanling rats

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:6994470

Flores, C A; Brannon, P M; Wells, M A; Morrill, M; Koldovsky, O ^[1]

---

1. Univ. of Arizona College of Medicine, Tucson (USA)

To determine the effect of altered dietary fat intake on the rate of fat absorption in the intact animal, we fed male weanling rats either a high fat-low carbohydrate (HF-LC) (calories: 67% fat, 10% carbohydrate, 20% protein) or low fat-high carbohydrate (LF-HC) (calories: 10% fat, 67% carbohydrate, 20% protein) diet for 8 days. Absorption of ({sup 14}C)triolein was estimated by determining (1) {sup 14}CO{sub 2} expiration in breath, (2) intestinal triglyceride output using Triton WR-1339, an inhibitor of lipoprotein lipase, and (3) quantitating the disappearance of labeled triolein from the gastrointestinal tract. Changes in the activity of pancreatic lipase and amylase confirmed the adaptation to altered fat and carbohydrate intake. Animals fed the HF-LC diet exhibited approximately twofold greater triolein disappearance, oxidation, and intestinal triglyceride output compared with animals fed LF-HC. There was also a highly significant linear relationship between {sup 14}CO{sub 2} excretion and intestinal triglyceride output in both diet groups. These data show that high dietary fat content markedly enhances in vivo fat absorption in the weanling rat.

• OSTI ID: 6994470
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 258; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMYLASE
ANIMALS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLESTERASES
CHALCOGENIDES
DIET
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPASES
LIPIDS
MAMMALS
METABOLISM
O-GLYCOSYL HYDROLASES
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PANCREAS
RATS
RODENTS
TRACER TECHNIQUES
TRIGLYCERIDES
TRIOLEIN
UPTAKE
VERTEBRATES