Measurement of the rate of fat absorption by the /sup 14/C-triolein breath test

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5388324

Flores, C; Bezerra, J; Wells, M; Koldovsky, O

To characterize the extent to which continuous measurement of the rate of /sup 14/CO/sub 2/ excretion following the oral administration of /sup 14/C-labeled triolein (/sup 14/C-T) would estimate the rate of absorption of triolein by the gut, ten adult male rats were fed 0.5 ml of /sup 14/C-T and the rate of production of expired labeled CO/sub 2/ was measured continuously. Pairs of animals were sacrificed at 30 min intervals between 90 and 210 min and absorption rates were calculated by quantitating substrate remaining in the gut. Comparison of these rates with the cumulative excretion rates of labeled CO/sub 2/ revealed a linear relationship with a correlation coefficient of 0.91. In a second experiment, adult rats received an intravenous injection of Triton WR-1339, a potent inhibitor of lipoprotein lipase, prior to receiving /sup 14/C-T. The rate of intestinal /sup 14/C-triglyceride output was determined from the increase in /sup 14/C in the blood over a 6 hr period. Comparison of the rates of intestinal secretion using Triton WR-1339 with that determined from the cumulative rates of excretion of /sup 14/CO/sub 2/ revealed a correlation coefficient of 0.98. The authors conclude that rates of triglyceride absorption can be estimated in vivo by the continuous measurement of labeled CO/sub 2/ excreted in breath.

• Research Organization: Univ. of Arizona, Tucson
• OSTI ID: 5388324
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CLEARANCE
ENZYME INHIBITORS
ESTERS
EXCRETION
IN VIVO
INJECTION
INTAKE
INTESTINAL ABSORPTION
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
OILS
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RATS
RODENTS
TRACER TECHNIQUES
TRIGLYCERIDES
TRIOLEIN
UPTAKE
VERTEBRATES