Interaction of electromagnetic fields with chondrocytes in gel culture. Final report, 15 Jan 92-14 Jan 94

Technical Report · Mon Jan 13 23:00:00 EST 1992

OSTI ID:6991920

Grodzinsky, A J; Buschmann, M D; Gluzband, Y A

The specific objectives of this research period were: (1) to quantify the effect of applied electric fields on chondrocyte metabolism, using a range of stimulation frequencies and amplitudes; (2) to compare the chondrocyte biosynthetic response to applied fields at early times in agarose gel culture before an extracellular matrix has accumulated and at later times after significant deposition of matrix around and between the cells; and (3) to begin to interpret the biosynthetic response to applied fields in terms of models of physical mechanisms. The results of these studies suggest that electric fields applied to chondrocytes in agarose can modulate the synthesis of proteoglycans and protein constituents. Biosynthesis may be inhibited or stimulated depending on the amplitude of the applied current density. In addition, the presence of extracellular matrix may enhance the ability of normal chondrocytes and cells in intact cartilage to respond to electric fields, although the presence of matrix was not required for the stimulatory response to be observed with Swarm rat chondrosarcoma cells.

• Research Organization: Massachusetts Inst. of Tech., Cambridge, MA (United States). Dept. of Electrical Engineering and Computer Science
• OSTI ID: 6991920
• Report Number(s): AD-A-253727/2/XAB; CNN: AFOSR-91-0153
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARTILAGE
COLLOIDS
CONNECTIVE TISSUE
DISPERSIONS
ELECTROMAGNETIC FIELDS
GELS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
PROTEINS
RATS
RODENTS
SENSITIVITY
SYNTHESIS
TISSUES
VERTEBRATES