Stimulation of DNA synthesis by ascorbate in cultures of articular chondrocytes
The addition of 0.2 mM Na L-ascorbate increased the incorporation of 3H-thymidine by rabbit articular chondrocytes in cell and organ culture. The stimulatory response of explants to ascorbate was potentiated by pretreatment of the cartilage with 0.2% clostridial collagenase (type 1) or trypsin for 15-30 minutes. In explants there was a latent period of 3 to 4 days before increased labeling of the nuclei could be detected. The effect was transient and declined after 8 days of culture. It was more evident in organ cultures of immature (3-month-old) than 2- to 3-year-old rabbits. Age differences were not detected in cell cultures. Explants of adult human articular cartilage were stimulated by ascorbate when the medium was supplemented with 10% fresh human serum but not by fetal bovine serum. The findings indicated that synthesis of DNA by articular chondrocytes in situ is regulated by responsiveness of the cells proper to compounds such as vitamin C, by properties of the extracellular matrix, and by factors in the serum. Ascorbate was cytotoxic at concentrations greater than 0.2 mM in the presence of certain batches of serum.
- Research Organization:
- Department of Pathology, State University of New York at Stony Brook
- OSTI ID:
- 5136322
- Journal Information:
- Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 25:3; ISSN ARHEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ASCORBIC ACID
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARTILAGE
CELL CULTURES
CONNECTIVE TISSUE
DNA
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RABBITS
RIBOSIDES
SYNTHESIS
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMINS