Effects of iron deficiency on free radical scavenging enzymes in muscles of diabetic rats

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6989187

Morrow, S; Hegarty, P V.J.

Catalase, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) prevent free-radical mediated tissue damage. Diabetes increases, and low dietary intakes of iron decreases catalase activity in muscles. Therefore, the combined effects of diabetes and iron deficiency on the free radical enzyme scavenging system was studied. Male, weanling rats were injected with streptozotocin (65 mg/kg, I.V.) and fed diets containing either 35 ppm (Db+Fe) or 8 ppm (Db-Fe) iron. Sham-injected animals served as iron adequate (C+Fe) or iron deficient (C-Fe) controls. Heart, gastrocnemius, soleus and anterior tibialis muscles were dissected, weighed and analyzed for catalase, SOD and GSH-Px activities after 1,3 or 6 weeks on the respective diets. Muscles in Db+Fe and Db-Fe groups had elevated catalase activity after one week in the diabetic state. Conversely, catalase activity was depressed in the C-Fe animals. SOD and GSH-Px activities did not differ from control values for any experimental group. Treatment with insulin and/or iron returned catalase activity to control levels. These data indicate that iron deficiency does not inhibit responses of muscle catalase to the diabetic condition, and the diabetic condition exerts an effect on catalase which is independent of SOD and GSH-Px.

• Research Organization: Univ. of Texas Graduate School, Houston
• OSTI ID: 6989187
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550900 -- Pathology
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
CATALASE
DIABETES MELLITUS
DISEASES
ELEMENTS
ENDOCRINE DISEASES
ENZYME ACTIVITY
ENZYMES
HORMONES
INSULIN
IRON
MAMMALS
METABOLIC DISEASES
METALS
MUSCLES
NUTRITIONAL DEFICIENCY
OXIDOREDUCTASES
PATHOGENESIS
PEPTIDE HORMONES
PEROXIDASES
RADICALS
RATS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES