Effect of marginal zinc deficiency on the apoprotein (APO) B concentration of lymph chylomicrons
The concentration of apo B of lymph chylomicrons was determined in adult male rats fed 3 ppm of dietary zinc (ZD), as compared with pair-fed (PF) and ad-libitum fed (CT) controls given 30 ppm of zinc. Chylomicrons were isolated from the mesenteric lymph by ultracentrifugation and purified by 2% agarose column chromatography. Apo B of purified chylomicrons was separated by precipitation and subsequent washing with isopropanol. The results showed that the apo B content of chylomicrons was significantly decreased in ZD rats; the apo B contents in ZD, PF and CT groups, as expressed as % protein, were 8.7 +/- 0.1, 11.5 +/- 0.5 and 10.7 +/- 0.7, respectively. The ratios of apo B/triglyceride and apo B/chylomicron were significantly lower in ZD group, compared with those in PF and CT rats. However, no significant differences were observed between ZD and PF rats in the concentrations of total protein, triglyceride (TG), cholesterol (CH) and phospholipid, although there was an increase in CH and a slight decrease in TG in CT rats. The serum levels of zinc in ZD, PF and CT rats were 1.26 +/- 0.16, 1.89 +/- 0.16 and 1.86 +/- 0.04 ppm, respectively. The decrease in chylomicron apo B may provide an explanation for the impaired intestinal absorption and subsequent metabolism of ingested lipids in zinc deficiency.
- Research Organization:
- Oral Roberts Univ. School of Medicine, Tulsa, OK
- OSTI ID:
- 6989175
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMICAL COMPOSITION
CHOLESTEROL
CHROMATOGRAPHY
CHYLOMICRONS
DIET
ELEMENTS
ESTERS
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
LIPIDS
LYMPH
MAMMALS
MATERIALS
METALS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
STEROIDS
STEROLS
TRIGLYCERIDES
UPTAKE
VERTEBRATES
ZINC