Intestinal apoprotein biosynthesis: alpha-1-antitrypsin identified as a constituent of rat lymph chylomicrons
Apolipoproteins synthesized in the intestine can serve as a source of constituents for plasma lipoproteins and play an important role in the pathways of lipoprotein formation, interconversion, and catabolism. In the present study, apolipoproteins synthesized by the intestine were identified in mesenteric lymph following intraduodenal administration of lipid and (/sup 14/C)-leucine to rats. The study was undertaken to assess differences in the rate of appearance of newly synthesized apolipoproteins into chylomicron and VLDL particles of rat mesenteric lymph and to determine if there is a sex-related influence on this process. Examination of qualitative and quantitative differences in apolipoprotein profiles, (/sup 14/C)-leucine labeling patterns, and the specific activities of the individual apolipoproteins secreted with either chylomicrons or VLDL distinguish the two particles and their pathways of assembly. A protein of molecular weight 54,000 daltons was recovered with the chylomicrons. The protein was shown to be synthesized by rat liver hepatocytes, and immunoprecipitation studies with rat intestinal cells incubated with /sup 35/S-methionine included this organ as a source of alpha-1-antitrypsin.
- Research Organization:
- George Washington Univ., Washington, DC (USA)
- OSTI ID:
- 6281495
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
APOLIPOPROTEINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHYLOMICRONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HYDROLASES
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER CELLS
LYMPH
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METHIONINE
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SERINE PROTEINASES
SEX DEPENDENCE
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES