In vivo clearance of chylomicron triglycerides containing w-3 eicosapentaenoic acid

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5391865

Chen, I S; Satchithanandum, S; Cassidy, M M; Sheppard, A J; Vahouny, G V

The in vivo clearance of chylomicron triglycerides (TG) enriched in either oleic acid (OA) or w-3 eicosapentaenoic acid (EPA) was compared. Chylomicrons were obtained from the mesenteric lymph (0-6 h) of rats, administered either 0.3 mM (1-/sup 14/C) OA or (1-/sup 14/C) EPA as an intraduodenal aqueous emulsion. The chylomicrons enriched with (1-/sup 14/C) OA or (1-/sup 14/C) EPA were isolated and purified by ultracentrifugation, and were injected into the jugular vein of recipient anesthetized rats. There was no significant difference between the two groups when total serum radioactivity was compared at 2, 5, and 10 min. However, at 25, 60, and 90 min, the clearance of EPA-enriched chylomicrons was significantly slower (P < 0.05). At each time analyzed the distribution of (/sup 14/C) oleic acid and of EPA among serum lipoproteins was comparable. After 5 min of chylomicron infusion the d < 1.006 g/ml lipoproteins contained almost 90% of the injected chylomicron radioactivity, while by 240 min, this fraction retained 42% of the remaining isotope. These and earlier studies suggested that the absorption and chylomicron transport of EPA in the intestine is not unusual, and that clearance of EPA-enriched chylomicrons may be only slightly slower than those enriched with oleate.

• Research Organization: George Washington Univ., Washington, DC
• OSTI ID: 5391865
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BLOOD-PLASMA CLEARANCE
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRIFUGATION
CHYLOMICRONS
CLEARANCE
EICOSANOIC ACID
ESTERS
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRIGLYCERIDES
ULTRACENTRIFUGATION
VERTEBRATES