Effect of soy protein on swine intestinal lipoproteins
Hypocholesterolemic effect of soy protein appears to be the result of reduced cholesterol absorption and enhanced cholesterol excretion. The objective of this study is to delineate the underlying mechanism of soy protein effect on cholesterol absorption. At the end of a 5-week soy-protein or casein diet, swine were subjected to cannulation of mesenteric lymph duct under halothane anesthesia. A single dose of 250 ..mu..Ci (/sup 14/C)-cholesterol and 10 mCi (/sup 3/H)-leucine was infused into the upper jejunum two hours after one-fifth of daily food was given. Then lymph was collected hourly for three hours and the lipoprotein fractions were separated by ultracentrifugation. SDS-PAGE (5%) was used to measure the concentrations of individual apoproteins by densitometric scanning. The three-hour lymphatic transport of cholesterol in casein-fed swine was significantly higher than in those fed soy protein. Triglyceride transports were similar in two groups. The (/sup 3/H)-leucine incorporation study revealed that transport of apo B-48 bore a significant positive relationship to transport of cholesterol in both chylomicron and VLDL fractions of mesenteric lymph. A greater apo B-48 secretion with higher specific activity was probably responsible for the greater transport of cholesterol in chylomicrons in casein-fed swine. On the other hand, the lesser cholesterol transport in chylomicrons in soy protein-fed swine was probably caused by lower apo B-48 secretion. Similarly, the transport of lymph VLDL cholesterol in swine fed casein or soy protein paralleled the amount of accompanying apo B-48. Dietary proteins probably influence the intestinal synthesis of apo B-48 which in turn affects cholesterol transport into the lymphatics.
- Research Organization:
- Albany Medical Coll., NY (USA)
- OSTI ID:
- 6924574
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMINO ACIDS
ANIMALS
APOLIPOPROTEINS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CASEIN
CHOLESTEROL
CHYLOMICRONS
CLEARANCE
DIET
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ESTERS
EXCRETION
FOOD
GASTROINTESTINAL TRACT
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
LIPOPROTEINS
LYMPH
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PLANTS
PROTEINS
RESPONSE MODIFYING FACTORS
SOYBEANS
STEROIDS
STEROLS
SWINE
SYNTHESIS
TRACER TECHNIQUES
TRIGLYCERIDES
TRITIUM COMPOUNDS
UPTAKE
VEGETABLES
VERTEBRATES