Structure of a rat hepatoma heparan sulfate

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6985636

Fedarko, N S; Ishihara, M; Conrad, H E

Previous studies showed that as monolayer cultures of a rat hepatocyte cell line passed from log growth to confluency there was an increase in sulfation of heparan sulfate (HS) and the accumulation of a unique species of HS with a high content of sulfated GlcA residues in the nucleus. The present study compares the HS metabolism of a rat (Morris) hepatoma line. Cells were labeled with /sup 35/SO/sub 4//sup 2 -/ and the structure and distribution of (/sup 35/SO/sub 4/)HS from the culture medium (CM), the pericellular matrix (Ma), the nucleus (NUC), the outer nuclear membrane (NM), and the remaining cytoplasmic (CP) pool was measured as nitrous acid-susceptible material. The amount of label incorporated into each pool was 1/10 that observed in the hepatocyte line. The HS proteglycan and the free HS chains from the hepatoma showed size distributions similar to those found for the hepatocytes, but a lower average charge density. In the HS from the CM, Ma, and CP pools 56% of glucosamine residues were sulfated; in that from the NM and NUC pools 46% were sulfated. HONO treatment gave mono- and disulfated disaccharides in a ratio of 1.5:1 for all five cellular pools, but showed that the HS from the NUC pool did not contain high levels of sulfated GlcA residues.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 6985636
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEPARIN
HEPATOMAS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MAMMALS
MEMBRANES
METABOLISM
MOLECULAR STRUCTURE
MUCOPOLYSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES