Transport of heparan sulfate into the nuclei of hepatocytes

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7170665

Ishihara, M; Fedarko, N S; Conrad, H E

A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains enriched in GlcA-2-SO/sub 4/ residues in the nucleus was labeled with /sup 35/SO/sub 4//sup 2 -/ and the rate of appearance of (/sup 35/SO/sub 4/)HS in the nucleus was measured. (/sup 35/SO/sub 4/)HS began to accumulate in the nucleus 2 h after the addition of /sup 35/SO/sup 42 -/ and reached a steady state level after 20 h. HS was lost from the nuclei of prelabeled cells with a t/sub 1/2/ of 8 h. Chloroquine did not inhibit the transport of HS into the nucleus, but increased the t/sub 1/2/ for the exit of HS from the nucleus to 20 h. At both 37/sup 0/C and 16/sup 0/C exogenous (/sup 35/SO/sub 4/)proteoHS was taken up by the cells and converted to free chains and about 10% of the internalized (/sup 35/SO/sub 4/)HS was transported into the nucleus. The (/sup 35/SO/sub 4/)HS isolated from the nucleus was enriched in GlcA-2-SO/sub 4/ residues, whereas the (/sup 35/SO/sub 4/)HS remaining in the rest of the intra-cellular pool showed a corresponding depletion in GlcA-2-SO/sub 4/ residues. The results show that nuclear HS is derived from the pool of a secreted proteoHS and that metabolism of exogenous HS by hepatocytes does not involve lysosomal processing of the internalized HS.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 7170665
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEPARIN
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES