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Metabolism of heparan sulfate proteoglycan in rat hepatocyte cell line and its role for regulation of cell growth

Thesis/Dissertation ·
OSTI ID:5471490
A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains in the nucleus was labeled with {sup 35}SO{sub 4}{sup 2{minus}} and the rate of appearance of ({sup 35}SO{sub 4})HS in the nucleus was measured. ({sup 35}SO{sub 4})HS began to accumulate in the nucleus 2 h after the addition of {sup 35}SO{sub 4}{sup 2{minus}} and reached a steady state level after 20 h. HS was lost from the nuclei of pre-labeled cells with a t{sub 1/2} of 8 h. At both 37{degree}C and 16{degree}C exogenous ({sup 35}SO{sub 4}) heparan sulfate proteoglycan (HSPG) was taken up by the cells and converted to free chains and about 10 percent of the internalized HS was transported into nucleus. The core protein of newly secreted HSPG appears in the cell matrix covalently linked to a phosphatidylinositol moiety on plasma membrane which is then cleaved by a plasma membrane phospholipase C. The released HSPG becomes found to a inositol-PO{sub 4} (IP)-specific receptor and is internalized and a portion of it is processed by a non-lysosomal pathway before delivery to the nucleus. Insulin activates the activity of the plasma membrane phospholipase C.
Research Organization:
Illinois Univ., Urbana, IL (USA)
OSTI ID:
5471490
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
EVEN-ODD NUCLEI
FUNCTIONS
GLYCOPROTEINS
GROWTH
HORMONES
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPASE
LIVER CELLS
MAMMALS
MEMBRANES
METABOLISM
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES