Correlation of heparan sulfate proteoglycan structure and metabolism with the regulation of cell division
Thesis/Dissertation
·
OSTI ID:6904898
Heparan sulfate proteoglycan (HSPG), a ubiquitous cell surface component in animal cells, is a complex, polyanionic macromolecule consisting of a core protein to which are attached several heparan sulfate (HS) chains. Growing and confluent cultures of a rat hepatocyte cell line were labeled with /sup 35/SO/sub 4//sup 2 -/ and the HS in the culture medium, the pericellular matrix, the nucleus, the outer nuclear membrane, and the remaining cytoplasmic pool was purified by DEAE-cellulose chromatography. The HS in all pools from the confluent cells exhibited a higher average charge density that the corresponding pools from the growing cells. Analysis of the mixtures of di- and tetrasaccharides formed by cleavage of HS with nitrous acid showed that the structural features of the HS in each pool were different and were altered significantly when growing cells became confluent.
- Research Organization:
- Illinois Univ., Urbana (USA)
- OSTI ID:
- 6904898
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLYCOPROTEINS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MEMBRANES
METABOLISM
MOLECULAR STRUCTURE
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLYCOPROTEINS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MEMBRANES
METABOLISM
MOLECULAR STRUCTURE
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES