Arrest of the cell cycle of hepatocytes by treatment with heparan sulfate proteoglycan

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6374484

Fedarko, N S; Ishihara, M; Conrad, H E

A cell surface heparan sulfate proteoglycan (HSPG) released by treatment of a rat hepatocyte cell line with 2mM inositol-2-PO/sub 4/, was isolated from log and confluent cultures. At 1.0..mu..g/ml the HSPG from confluent cells, but not that from log phase cells, inhibited cell division when added to log phase cultures. After a time equal to one cell cycle, the treated cells resumed logarithmic growth. Continuous administration of HSPG prolonged the delay in cell division. When growth resumed, the cells grew to confluence and exhibited contact inhibition. Cultures were synchronized by a thymidine/aphidicolin block at the G1/S boundary. When HSPG was added with release of the block, the cells progressed through S and the first mitosis. However, the second round of DNA synthesis and the mitosis were delayed for a period equal to one cell cycle. Studies of the uptake of (/sup 35/SO/sub 4/)-HSPG by synchronized cells showed internalization and accumulation of HS in nuclei after release of the block and then disappearance of nuclear (/sup 35/SO/sub 4/)-HS at the first mitosis. During the lengthened G1 phase following mitosis, (/sup 35/SO/sub 4/)-HS reappeared in the nuclei. A similar pattern of cell cycle dependent appearance and disappearance of nuclear HS was observed in studies of /sup 35/SO/sub 4//sup 2 -/ incorporation into HSPG by synchronized cells.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 6374484
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CARBOHYDRATES
CELL CONSTITUENTS
CELL CYCLE
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
EVEN-ODD NUCLEI
FUNCTIONS
GLUCOPROTEINS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MAMMALS
NUCLEI
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES