Inhibition by 2-deoxy-D-ribose of DNA synthesis and growth in Raji cells
Journal Article
·
· Proc. Soc. Exp. Biol. Med.; (United States)
When Raji cells were cultured for 3 days in serum-free medium, addition of 2-deoxy-D-ribose at the start of culture inhibited incorporation of (/sup 3/H)thymidine and cell division. At deoxyribose concentrations between 1 and 5 mM, viability was 80% or greater after 3 days of culture even though 5 mM deoxyribose inhibited thymidine incorporation 95-99%. Inhibition by deoxyribose could be completely reversed if the culture medium was replaced with fresh medium up to 8 hr after the start of culture. The inhibition was specific for deoxyribose since other monosaccharides had no effect. Inhibition of DNA synthesis did not appear to be due to depletion of essential nutrients in the medium since the percentage inhibition of thymidine incorporation by cells cultured either in suboptimal serum-free media or in media supplemented with 0.025-5% human AB serum was similar. When DNA repair synthesis was measured as hydroxyurea-resistant thymidine incorporation, addition of deoxyribose to Raji cultures caused increased thymidine incorporation. These results, together with data from others,suggest that deoxyribose damages DNA.
- Research Organization:
- Boston Veterans Administration Medical Center, MA (USA)
- OSTI ID:
- 6956494
- Journal Information:
- Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 187:4; ISSN PSEBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CONNECTIVE TISSUE CELLS
DEOXYRIBOSE
DNA REPLICATION
GROWTH
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSES
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CONNECTIVE TISSUE CELLS
DEOXYRIBOSE
DNA REPLICATION
GROWTH
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSES
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
THYMIDINE
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE