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D-ribose inhibits DNA repair synthesis in human lymphocytes

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
; ; ; ;
D-ribose is cytotoxic for quiescent human lymphocytes and severely inhibits their PHA-induced proliferation at concentrations (25-50 mM) at which other simple sugars are ineffective. In order to explain these effects, DNA repair synthesis was evaluated in PHA-stimulated human lymphocytes treated with hydroxyurea and irradiated. D-ribose, in contrast to other reducing sugars, did not induce repair synthesis and therefore did not apparently damage DNA in a direct way, although it markedly inhibited gamma ray-induced repair. Taking into account that lymphocytes must rejoin physiologically-formed DNA strand breaks in order to enter the cell cycle, we suggest that D-ribose exerts its cytotoxic activity by interfering with metabolic pathways critical for the repair of DNA breaks.
Research Organization:
Istituto di Istologia ed Embriologia Generale Bologna, Italy
OSTI ID:
5016377
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMIDES
ANIMAL CELLS
ARABINOSE
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DNA REPAIR
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
INHIBITION
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSE
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
STRAND BREAKS
THYMIDINE
TOXICITY