Interaction of u. v. - and methyl methanesulfonate-induced DNA repair synthesis: a role for poly(ADP-ribose)
Journal Article
·
· Carcinogenesis (N.Y.); (United States)
Treatment of a human lymphoblastoid line (GM606) with methyl methanesulfonate (MMS) or N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) initially stimulates and then drastically inhibits repair synthesis. This inhibition is overcome and repair appears to be stimulated by the poly(ADP-ribose) synthetase inhibitor 3-aminobenzamide (3-AmB). The repair activity of older cultures is much diminished compared to those rapidly dividing, but the 3-AmB effect is seen in both 2- and 7-day cultures. The action of 3-AmB is very rapid and its removal results in a diminution of repair synthesis within 30 min. U.v.-induced repair synthesis was not affected by 3-AmB. However, the level of u.v.-induced repair synthesis was drastically reduced by treatment of cells with MMS. This MMS inhibition of u.v.-induced repair synthesis was completely counteracted by the presence of 3-AmB. Insofar as the effective action of 3-AmB is on poly(ADP-ribose) synthesis, the data suggest that activation of poly(ADP-ribose) synthesis by alkylation of DNA reduces the NAD and therefore the ATP level in the lymphoblastoid cells used. Reduced ATP levels result in a lessened ability to carry out u.v.-induced repair synthesis.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 6799514
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 5:5; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:6241557
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Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
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Mon Dec 31 23:00:00 EST 1984
· Radiat. Res.; (United States)
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OSTI ID:6137637
Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATION
ANIMAL CELLS
ANIMALS
ATP
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
CHEMICAL REACTIONS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ESTERS
INHIBITION
MAMMALS
MAN
METHYL METHANESULFONATE
MUTAGENS
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SULFONIC ACID ESTERS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATION
ANIMAL CELLS
ANIMALS
ATP
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
CHEMICAL REACTIONS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ESTERS
INHIBITION
MAMMALS
MAN
METHYL METHANESULFONATE
MUTAGENS
NITROSO COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SULFONIC ACID ESTERS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES