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Do inhibitor studies demonstrate a role for poly(ADP-ribose) in DNA repair

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576300· OSTI ID:6137637
; ;

3-Aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis, has been commonly used in attempts to demonstrate a regulatory role for the polymer during a late stage of repair. When a range of inhibitor concentrations was used paradoxical results were obtained. Up to 1 mM, 3-aminobenzamide appeared to reduce DNA break frequencies in cells damaged by methyl methane sulfonate; at doses of 2 mM and above, it appeared to increase break frequencies. In the high concentration range, many nonspecific side effects and cellular toxicity predominate. Evidence used to assert a role for poly(ADP-ribose) synthesis during ligation has usually been derived from experiments using high concentrations of 3-aminobenzamide, but these may be attributed to toxic side effects. The cellular effects of 3-aminobenzamide are therefore complicated by nonspecific effects over a commonly used concentration range and evidence for a specific regulatory role of poly(ADP-ribose) in DNA repair is weak.

Research Organization:
Univ. of California, San Francisco
DOE Contract Number:
AC03-76SF01012
OSTI ID:
6137637
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CONNECTIVE TISSUE CELLS
DNA REPAIR
ENZYME INHIBITORS
ESTERS
FIBROBLASTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METHYL METHANESULFONATE
MONOSACCHARIDES
MUTAGENS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSES
RECOVERY
REPAIR
RIBOSE
SACCHARIDES
SOMATIC CELLS
STRAND BREAKS
SULFONIC ACID ESTERS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS