Determination of thymidine in serum used for cell culture media
Journal Article
·
· Exp. Cell Biol.; (United States)
Thymidine concentrations in serum used for cell culture media were determined with an assay based on isotope dilution. In this assay, incorporation of (3H)-thymidine into DNA of cultured cells was measured in the presence of 5 and 20% serum as a function of the concentration of unlabeled thymidine added to the medium. Thymidine concentrations were measured using horse serum as well as fetal calf serum in the culture media. Dialysis of serum resulted in a reduction of thymidine levels by factors of at least 10.
- Research Organization:
- Univ. of Berne, Switzerland
- OSTI ID:
- 5061980
- Journal Information:
- Exp. Cell Biol.; (United States), Journal Name: Exp. Cell Biol.; (United States) Vol. 46:1-2; ISSN ECEBD
- Country of Publication:
- United States
- Language:
- English
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LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES
Journal Article
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Tue Dec 31 23:00:00 EST 1974
· In Vitro; (United States)
·
OSTI ID:7313451
Degradation or consumption of exogenous thymidine in absence or presence of exogenous deoxycytidine: Effect on DNA chain elongation and (/sup 3/H)thymidine incorporation in control and uv-irradiated CHO-K1 cells
Technical Report
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Mon Mar 31 23:00:00 EST 1986
·
OSTI ID:5673087
LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES
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Sat Feb 29 23:00:00 EST 1964
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OSTI ID:4078905
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZINES
BIOSYNTHESIS
BLOOD SERUM
CELL CULTURES
CULTURE MEDIA
DIALYSIS
DNA
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
59 BASIC BIOLOGICAL SCIENCES
AZINES
BIOSYNTHESIS
BLOOD SERUM
CELL CULTURES
CULTURE MEDIA
DIALYSIS
DNA
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE