Degradation or consumption of exogenous thymidine in absence or presence of exogenous deoxycytidine: Effect on DNA chain elongation and (/sup 3/H)thymidine incorporation in control and uv-irradiated CHO-K1 cells
Technical Report
·
OSTI ID:5673087
When CHO-K1 cells monolayers are grown in Ham's F-12 culture medium containing 10% fetal calf serum (medium A) exogenous thymidine (dThd) is degraded to thymine by a putative dThd phosphorylase. Thymine is then poorly incorporated into cellular DNA. When 2 mM deoxycytidine (dCyd) is added to medium A (medium B) no degradation of exogenous dThd occurs; rather dThd is consumed in the synthesis of DNA, presumably via a dThd kinase or other nucleoside salvage pathway. Differences in the kinetics of DNA synthesis, measured by (/sup 3/H)dThd pulse-incorporation or by alkaline sucrose velocity sedimentation, are observed in the two media. In comparison to cells in medium A, DNA chain elongation rates of cells in medium B are faster but total DNA synthesis in these cells, measured by incorporation of (/sup 3/H)dThd, appears to be 2- to 3-fold more sensitive to the inhibitory effects of 10 Jm/sup -2/ uv-radiation. 23 refs., 3 figs.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA); Northwest Coll. and Univ. Association for Science, Richland, WA (USA)
- DOE Contract Number:
- AC06-76RL01830; FG06-86ER75235
- OSTI ID:
- 5673087
- Report Number(s):
- PNL-SA-13956; ON: DE88005628
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBON 14 COMPOUNDS
CHO CELLS
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
550501 -- Metabolism-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBON 14 COMPOUNDS
CHO CELLS
DNA REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION