Simultaneous inhibition of thymidine degradation and stimulation of incorporation into DNA by 5-fluorodeoxy-uridine
Journal Article
·
· Plant Sci. Lett., v. 5, pp. 157-162
Cultured plant cells degrade exogenously supplied thymidine. 5-Fluorode- oxyuridine (FdUrd) can be used to inhibit this degradation while at the same time enhancing thymidine (dThd) incorporation into DNA. If $sup 14$C- or $sup 3$H- labeled dThd is used, the improvement in DNA labeling which results permits detection of DNA synthesis in freshly isolated protoplasts, as well as other sensitive measures of DNA metabolism in plant cells. (auth)
- Research Organization:
- Oak Ridge National Lab., TN
- NSA Number:
- NSA-33-019755
- OSTI ID:
- 4096782
- Journal Information:
- Plant Sci. Lett., v. 5, pp. 157-162, Journal Name: Plant Sci. Lett., v. 5, pp. 157-162; ISSN PTSLA
- Country of Publication:
- Netherlands
- Language:
- English
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Related Subjects
*CARBON 14-- CHEMICAL REACTIONS
*DNA-- LABELLING
*TRITIUM-- CHEMICAL REACTIONS
400702* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
BIODEGRADATION
LABELLED COMPOUNDS
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
NUCLEOSIDES
THYMIDINE
URACILS
*DNA-- LABELLING
*TRITIUM-- CHEMICAL REACTIONS
400702* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
BIODEGRADATION
LABELLED COMPOUNDS
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
NUCLEOSIDES
THYMIDINE
URACILS