Incorporation of deoxyuridine monophosphate into DNA increases the sister-chromatid exchange yield

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90023-1· OSTI ID:6791580

Pardo, E G; Hernandez, P; Gutierrez, C

The effect of a treatment with 5-fluoro-2'-deoxyuridine (FdUrd) in combination with 2'-deoxyuridine (dUrd) on cell proliferation, incorporation of DNA precursors into DNA and sister-chromatid exchanges (SCEs) has been analyzed in Allium cepa meristem cells. FdUrd in the range 10/sup -9/-5 x 10/sup -7/ M produced a dose- and time-dependent decrease in the amount of cells in mitosis. This inhibitory effect could be reversed by 70-80% in short-term (6 h) experiments, by exogenously supplied dUrd at a concentration of 10/sup -1/ M. However, at the highest FdUrd dose tested (10/sup -7/ M), 10/sup -4/ M dUrd could not reverse the FdUrd effect in long-term experiments as shown by analyzing the kinetics of synchronous cell populations. DNA extracted from cells pulsed with (6-/sup 3/H)dUrd in the presence of FdUrd and 6-amino-uracil (6-AU), an inhibitor of uracil-DNA glycosylase, contained a small amount of label in the form of (6-/sup 3/H)dUMP. Thus the authors conclude that under the experimental conditions, exogenously supplied dUrd may be metabolized intracellularly to 2'-deoxyuridine triphosphate (dUTP) and that this deoxynucleotide may eventually be mis-incorporated into DNA. By analyzing SCE levels in third division chromosomes of cells treated with FdUrd and dUrd during their second cycle, they has scored a 6-fold increase in the reciprocal SCE level which demonstrates that the replication of a dUMP-containing DNA template leads to a higher SCE yield.

• Research Organization: Universidad de Alcala de Henares, Madrid, Spain
• OSTI ID: 6791580
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 168:2; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALLIUM CEPA
ANTI-INFECTIVE AGENTS
ANTIMETABOLITES
ANTIMICROBIAL AGENTS
AZINES
BIOLOGICAL EFFECTS
BODY
CELL DIVISION
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
DEOXYURIDINE
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
FLUOROURACILS
FUDR
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MERISTEMS
MITOSIS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT TISSUES
PLANTS
PYRIMIDINES
RADIOSENSITIZERS
RIBOSIDES
SISTER CHROMATID EXCHANGES
TISSUES
TRITIUM COMPOUNDS
URACILS