Study of the antimetabolite properties of anomeric 5-substituted 2'-deoxyuridines

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Pharmaceut. Chem. J.; (United States)

OSTI ID:6270767

Nedorezova, T P; Potapova, G I; Mel'nik, S Ya; Preobrazhenskaya, M N

The authors study the influence of anomeric 5-substituted 2'-dioxyuridines compounds I-XIV on the incorporation of labeled precursors into the DNA of ascites hepatoma 22A cells of C3HA mice in vitro and in vivo. In addition, the DNA of chicken fibroblasts, growing in a monolayer, before and after infection with herpes simplex virus was studied. The influence of the given compounds I-XIV on the activity of thymidine and deoxycytidine kinases and pyrimidine nucleoside phosphorylases, isolated and partially purified from tumor cells of animals and humans was also investigated. In some of the experiments, radioactive precursors /sup 14/C-dThd and /sup 3/H-dCyd or (/sup 3/H)deoxyuridine were injected. The kinetics of the competitive phosphorylation of /sup 14/C-dThd in the presence of antiherpetic nucleosides and the phosphorylation of these compounds with gamma-/sup 32/P-ATP as the second substrate of the reaction were studied.

• Research Organization: All-Union Oncologic Research Center, Moscow (USSR)
• OSTI ID: 6270767
• Journal Information: Pharmaceut. Chem. J.; (United States), Journal Name: Pharmaceut. Chem. J.; (United States) Vol. 20:6; ISSN PCJOA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMETABOLITES
ANTINEOPLASTIC DRUGS
ASCITES TUMOR CELLS
AZINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIRDS
CARBON 14 COMPOUNDS
CHEMISTRY
CHICKENS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
CYTIDINE
CYTOCHEMISTRY
DISEASES
DRUGS
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
FOWL
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MATERIALS TESTING
METABOLISM
MICE
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIDINES
PYRIMIDINES
RADIOENZYMATIC ASSAY
RIBOSIDES
RODENTS
SCINTILLATION COUNTING
SKIN DISEASES
SOMATIC CELLS
TESTING
THYMIDINE
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS
URACILS
VERTEBRATES
VIRAL DISEASES
VIRUSES