Radioimmunoassays of plasma thymidine, uridine, deoxyuridine, and cytidine/deoxycytidine
Journal Article
·
· Anal. Biochem.; (United States)
Radioimmunoassay techniques have been developed for the assay of thymidine, uridine, deoxyuridine, and deoxycytidine. Plasma levels of the first three nucleosides have been measured, and an upper limit has been determined for the plasma concentration of deoxycytidine. The assays involve displacement of a (3H)pyrimidine nucleoside from the appropriate labeled rabbit immunoglobulin. By assaying a mixture of uridine and deoxyuridine in the presence and absence of borax, the concentrations of both nucleosides have been measured. In seven healthy adults, plasma levels of uridine were 21.1 +/- 8.4 ..mu..M (mean +/- SD) and of deoxyuridine were 0.62 +/- 0.39 ..mu..M. In cancer patients, thymidine levels were 7.5 +/- 2.7 x 10/sup -7/M. The upper limit for plasma deoxycytidine levels in six healthy adults was 0.71 +/- 0.1 ..mu..M.
- Research Organization:
- Univ. of Sydney, New South Wales, Australia
- OSTI ID:
- 5574457
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 115:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANTIMETABOLITES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
BORATES
BORAX
BORON COMPOUNDS
DATA
DEOXYCYTIDINE
DEOXYURIDINE
DISEASES
DRUGS
EXPERIMENTAL DATA
GLOBULINS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PATIENTS
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RIBOSIDES
SODIUM COMPOUNDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANTIMETABOLITES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
BORATES
BORAX
BORON COMPOUNDS
DATA
DEOXYCYTIDINE
DEOXYURIDINE
DISEASES
DRUGS
EXPERIMENTAL DATA
GLOBULINS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PATIENTS
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RIBOSIDES
SODIUM COMPOUNDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE