LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES
Experiments were performed with C/sup 14/-thymidine or unlabeled thymidine added to HeLa S3 cell cultures growing in Eagle's medium. The results show that thymidine in the culture medium causes a concentration-dependent inhibition of growth, with effects being significant at levels as low as 10 mu g/ ml, thymidine in the medium reduces the incorporation of H/sup 3/-uridine into both DNA and RNA, the effect on incorporation into RNA being unexpectedly great, and other nucleosides, added singly or together to the medium, cause effects similar to those induced by thymidine. Thirty-minute incubations with tritiated thymidine, at concentrations from 0.02 to 2.5 mu C/ml, and at two specific activities, did not affect the production of colonies of HeLa S3 cultures. (auth)
- Research Organization:
- Ames Research Center, Moffett Field, Calif. and Brookhaven National Lab., Upton, N.Y.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-015802
- OSTI ID:
- 4078905
- Journal Information:
- Radiation Res., Journal Name: Radiation Res. Vol. Vol: 21
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Further Studies on the Clonal Growth of HeLa S3 Cells Treated with Tritiated Thymidine
ACTION OF TRITIATED THYMIDINE ON THE CLONAL GROWTH OF MAMMALIAN CELLS
Determination of thymidine in serum used for cell culture media
Journal Article
·
Wed Feb 28 23:00:00 EST 1962
· Radiation Research
·
OSTI ID:4835691
ACTION OF TRITIATED THYMIDINE ON THE CLONAL GROWTH OF MAMMALIAN CELLS
Journal Article
·
Thu Oct 01 00:00:00 EDT 1959
· Radiation Research
·
OSTI ID:4234073
Determination of thymidine in serum used for cell culture media
Journal Article
·
Sat Dec 31 23:00:00 EST 1977
· Exp. Cell Biol.; (United States)
·
OSTI ID:5061980