ACTION OF TRITIATED THYMIDINE ON THE CLONAL GROWTH OF MAMMALIAN CELLS

Journal Article · Thu Oct 01 04:00:00 EDT 1959 · Radiation Research

DOI:https://doi.org/10.2307/3570807· OSTI ID:4234073

Drew, R M; Painter, R B

S>The effect of intracellular incorporation of tritiated thymidine (H/ sip 3/TDR) on mammalian cell cytology and survival has been investigated by the single-cell plating technique. A survival curve of HeLa S3 cells grown in medium containing H/sub 3/TDR has been constructed which demonstrates the lethal action of the radioisotope. At concentrations of 0.1 f UO/sub 2/ c/ml or greater, very few cells survive and continue to divide to form macroscopic colonies in the plate cultures. The lethal action of H/sup 3/TDR has been shown to be a function of both the dose and the specific activity. The cytological appearance of the cells is changed as a result of exposure to H/sup 3/TDR. Giant cell formation, loosely packed cells, and abortive colonial growth all indicate a radiation effect. No reversal of the killing effect could be demonstrated when H/sup 3/TDR- treated cells were grown over nonmultiplying, x-irradiated feeder cells. (auth)

• Research Organization: Brookhaven National Lab., Upton, N.Y.
• Sponsoring Organization: USDOE
• NSA Number: NSA-13-021886
• OSTI ID: 4234073
• Journal Information: Radiation Research, Journal Name: Radiation Research Vol. Vol: 11
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
CANCER
CYTOLOGY
HETEROCYCLICS
LABELLED COMPOUNDS
LETHAL DOSE
METABOLISM
MITOSIS
NUCLEIC ACIDS
NUCLEOSIDES
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
QUANTITY RATIO
RADIATION INJURIES
SURVIVAL TIME
THYMIDINE
THYMINE
TRITIUM
X RADIATION