Further Studies on the Clonal Growth of HeLa S3 Cells Treated with Tritiated Thymidine
The effect of intranuclear incorporation of H/sup 3/-thymidine on the clonal growth of HeLa S3 cells was studied, and the supression of colony development was shown to be a function of the variability of the uptake of the radioisotope by cells within clones. Autoradiograms showed that in young cultures the percentsge of clones in which all cells were labeled after a 24-hr growth period in medium containing 0.1 mu C of H/sup3 /-thymidine per milliliter was much greater than in older cultures having an average of about forty cells per clone. When similar cultures were permitted to incubate in nonradioactive medium after the growth period in radioactive medium, there was a greater suppression of colony development in the younger cultures than in those with more cells per clone. Variability in the uptake of H/sup 3/-thymidine by cells within clones was demonstrated to be a function of the degree of variability in the generative cycle of cells. A small fraction of cells appears to have a somewhat longer generation time than the average (22 to 24 hr). If the incubation time in the radioactive medium is extended to 48 hr, these cells incorporate the radioisotope, and colony development is completely suppressed. Survival curves for S3 cells cultured in varying concentrations of H/sup 3/-thymidine for 24 and 48 hr were established for comparison. Both curves indicate that a concentration of H/sub 3/-thymidine of the order of 0.02 mu C/ml suppresses colony development. The lethal effect at this dose level is significant in the 48-hr treated cultures.
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-017512
- OSTI ID:
- 4835691
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 3 Vol. 16; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
ACTION OF TRITIATED THYMIDINE ON THE CLONAL GROWTH OF MAMMALIAN CELLS
LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES
Effect of cell passage on the susceptibility of BALB/3T3 clone A31-1-1 cells to 3-methylcholanthrene-induced morphological transformation
Journal Article
·
Thu Oct 01 00:00:00 EDT 1959
· Radiation Research
·
OSTI ID:4234073
LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES
Journal Article
·
Sat Feb 29 23:00:00 EST 1964
· Radiation Res.
·
OSTI ID:4078905
Effect of cell passage on the susceptibility of BALB/3T3 clone A31-1-1 cells to 3-methylcholanthrene-induced morphological transformation
Journal Article
·
Wed Dec 31 23:00:00 EST 1986
· Environ. Mutagen.; (United States)
·
OSTI ID:6934699