Improved methods for reducing calcium and magnesium concentrations in tissue culture medium: application to studies of lymphoblast proliferation in vitro

Journal Article · Wed Jan 01 04:00:00 EST 1975 · In Vitro; (United States)

DOI:https://doi.org/10.1007/BF02616371· OSTI ID:7313451

Brennan, J K; Mansky, J; Roberts, G; Lichtman, M A

We have compared several methods for reducing calcium and magnesium concentrations in tissue culture medium, with the objective of producing selective deficiency effects on the growth of mouse (L5178Y) and human (P1R) lymphoblasts. In experiments in which calcium- and magnesium-''free'' McCoy's medium was supplemented with 15 percent horse or fetal calf serum, enough calcium and magnesium were provided by serum to support normal lymphoblast growth rate. Either dialysis or chelating-resin treatment of horse or fetal calf serum reduced calcium and magnesium contents approximately 100-fold. Use of dialyzed sera resulted in reduced growth rate, although in most cases the reduction in growth could be attributed to other effects of dialysis on serum, inasmuch as growth in those experiments was not restored to normal by the addition of calcium and magnesium to the medium. In contrast, the reduction of lymphoblast growth rate that occurred when resin-treated serum was used was always attributable to removal of calcium and magnesium, as normal growth always occurred in cultures to which calcium and magnesium were added.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7313451
• Journal Information: In Vitro; (United States), Journal Name: In Vitro; (United States) Vol. 11:6; ISSN ITCSA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ABUNDANCE
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CELL PROLIFERATION
CHELATING AGENTS
CONNECTIVE TISSUE CELLS
ELEMENTS
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAGNESIUM
MAMMALS
MAN
METALS
MICE
PRIMATES
RODENTS
SOMATIC CELLS
TISSUE CULTURES
VERTEBRATES